地 点：光电国家研究中心 A101
报 告 人：Cai Ruiyao（蔡瑞瑶）, 德国慕尼黑大学卒中和痴呆研究所
邀 请 人：骆海明 教授
Cai Ruiyao（蔡瑞瑶）出生于中国浙江，5岁随父母搬到意大利米兰。 她在意大利米兰-比可卡大学于2011、2014年分别获得生物技术学士和硕士学位，其硕士论文研究软脑膜脑血管是如何影响脑中风。自2015年1月至今，她在德国慕尼黑大学卒中和痴呆研究所的Ali Ertürk博士实验室攻读博士学位，其研究方向是开发新的组织清除技术，以探索由创伤性脑损伤、脊髓损伤等脑损伤引发的变化。目前，Cai Ruiyao共发表SCI论文9篇，其中以第一作者在Nature Neuroscience、Nature Methods杂志上发表SCI论文各一篇。
Ruiyao Cai was born in Zhejiang, China. At the age of 5 she moved to Milan, Italy with her parents and here she grew up. She obtained her BSc and MSc in Biotechnology at the University of Milan-Bicocca, Italy and during her Master 's thesis she worked in the laboratory of Neurobiology on the role of leptomeningeal cerebral vessels in influencing the stroke outcome. After a period as intern at the University of Oxford and as research fellow in the lab of Neurobiology of the University of Milan-Bicocca, where she continued in the stroke field, she moved to Munich for her PhD studies in Neuroscience under the GSN graduate school. Currently, she is completing her doctoral program in Dr. Ali Ertürk's lab at the Institute for Stroke and Dementia Research, LMU, in Munich. Her research projects focus on the development of new tissue clearing technologies to explore changes triggered by brain injuries such as traumatic brain injury and spinal cord injury. Ruiyao has published over 10 refereed international papers in top journals including first-authored papers in Nature Neuroscience (2018) and Nature Methods (2016).
The effects of most diseases are not confined to a particular body region, therefore a systems biology approach is needed to study diseases at a whole-body level. Tissue clearing methods revolutionized standard histology, allowing the imaging of organs and organisms without sectioning. So far, the highest level of tissue transparency has been achieved by DISCO clearings. It has also been shown that they are compatible with antibody labeling. Nevertheless, owing to the fact that organic solvents can eventually quench the endogenous fluorescent signal, the reliable detection and quantification of the biological fluorescent information in all body districts have still represented a challenge. To overcome this issue, we developed vDISCO", which exploits a nanobody-based whole-body immunolabeling system to enhance the signal of fluorescent proteins, to preserve it permanently and to image through dense and hard tissues such as bones and skin. vDISCO allowed us to image and quantify subcellular details in intact transparent mice. Using vDISCO we visualized the first whole-body neuronal map in adult mice at subcellular resolution. Next, we screened whole mice for changes after CNS trauma and we found degeneration of peripheral nerve terminals in the torso. Moreover, using vDISCO we observed short vascular connections between skull marrow and meninges, which were filled with leucocytes upon stroke. Taken together, our method represents a powerful tool to analyze effects of diseases in whole organisms.