武汉光电论坛第139期

报告题目：生物医学中的化学成像：光学显微镜的下一个研究前沿

Chemical Imaging for Biomedicine: The Next Frontier of Light Microscopy

时 间：2018年5月28日10:30-12:00

地 点：光电国家研究中心A101

报 告 人：闵 玮 教授，美国哥伦比亚大学

邀 请 人：王 平 教授

报告人简介：

闵玮现任哥伦比亚大学化学系终身教授。2003年本科毕业于北京大学，2008年博士毕业于哈佛大学，师从美国科学院院士谢晓亮教授，2010年起执教哥伦比亚大学，2017年获得正教授职位。闵教授曾获的诸多奖项，包括2017美国化学会Early Career Award of Experimental Physical Chemistry、2017 Coblentz Award of Molecular Spectroscopy、2015 Buck-Whitney Award of ACS Eastern New York Section、2015 Camille Dreyfus Teacher-Scholar Award、2013 Alfred P. Sloan Research Fellowship等等。闵教授课题组的研究方向是应用生物分子光谱学去开发新型光学显微镜，结合化学探针和生物技术，推动神经科学、癌症检测和疾病诊断等前沿生命科学和医学课题的发展。闵教授已在Science、 Nature、Nature methods 和其它高影响力的刊物上发表了多篇学术论文。

Biography:

Dr. Wei Min graduated from Peking University with a Bachelor's degree of Chemistry in 2003. He received his Ph.D. from Harvard University in 2008 studying single-molecule biophysics with Prof. Sunney Xie. After continuing his postdoctoral work in Xie group, Dr. Min joined the faculty of Department of Chemistry at Columbia University in 2010, and is promoted to Full Professor there in 2017. He is also affiliated with the Kavli Institute for Brain Science at Columbia University. Dr. Min's contribution has been recognized by a number of honors, including: 2017 American Chemical Societies(ACS) Early Career Award of Experimental Physical Chemistry、2017 Coblentz Award of Molecular Spectroscopy、2015 Buck-Whitney Award of ACS Eastern New York Section、2015 Camille Dreyfus Teacher-Scholar Award、2013 Alfred P. Sloan Research Fellowship. Dr. Min's current research interests focus on developing novel optical spectroscopy and microscopy technology to address biomedical problems. In particular, his group has made important contributions to the development of stimulated Raman scattering (SRS) microscopy and its broad application in biomedical imaging including bioorthogonal chemical imaging of small molecules and super-multiplex vibrational imaging. Professor Wei has published many scientific papers on Science, Nature, Nature methods and other high impact journals.

报告摘要：

光学显微镜的技术革新将极大地改变生物学的研究方式。虽然荧光显微成像是目前细胞成像的首选方法，但在“组学”时代，它受到荧光基团过大，无法多色成像的限制。这里，我将提出两种化学成像策略。首先，我们结合新兴的受激拉曼散射显微镜与小的拉曼探针（例如炔烃、包括²H和¹³C等同位素）对无法进行荧光标记的生物小分子进行成像，研究脂肪酸代谢相关的脂肪毒性，葡萄糖摄取和代谢，药物传输，脑内蛋白质合成，DNA复制，蛋白质降解，RNA合成和肿瘤代谢。其次，我们发明了一种基于新型染料组成的振动调色板多色标记技术，开发了近共振受激拉曼散射显微镜。使用这种方法，我们观察了培养神经元和脑组织中的DNA和蛋白质代谢。这种多色成像方法有望促进复杂生物系统中相互作用研究，并在光学和生物技术中找到更广泛的应用。

Abstract:

Innovations in light microscopy have tremendously revolutionized the way researchers study biological systems. Although fluorescence microscopy is currently the method of choice for cellular imaging, it faces fundamental limitations such as the bulky fluorescent tags and limited multiplexing ability in the era of “omics”. In this talk, I will present two chemical imaging strategies, respectively. First, we devised a live-cell Bio-orthogonal Chemical Imaging platform suited for probing the dynamics of small bio-molecules, which can not be effectively labeled by bulky fluorophores. This scheme couples the emerging stimulated Raman scattering microscopy with tiny and Raman-active vibrational probes (e.g., alkynes, nitriles and stable isotopes including ²H and ¹³C). Exciting biomedical applications such as imaging fatty acid metabolism related to lipotoxicity, glucose uptake and metabolism, drug trafficking, protein synthesis in brain, DNA replication, protein degradation, RNA synthesis and tumor metabolism will be presented. Second, we invented a super-multiplex optical imaging technique. We developed electronic pre-resonance stimulated Raman scattering (epr-SRS) microscopy, achieving exquisite vibrational selectivity with high versatility and sensitivity. Chemically, we created a unique vibrational palette consisting of novel dyes bearing conjugated and isotopically-edited triple bonds, each displaying a single epr-SRS peak in the cell-silent spectra window. Up to 24 resolvable colors are currently achieved with great potential for further expansion. Using this approach, we monitored DNA and protein metabolism in neuronal co-cultures and brain tissues. This super-multiplex optical imaging approach promises to facilitate untangling the intricate interactions in complex biological systems, and find broad applications in photonics and biotechnology in general.

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