Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy

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Brian C. Wilson

Department of Medical Biophysics, University of Toronto/University Health Network, Toronto, Ontario M5G 1L7, Canada

Michael S. Patterson

Juravinski Cancer Centre and McMaster University, Hamilton, Ontario L8V 5C2, Canada

Buhong Li

MOE Key Laboratory of OptoElectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian 350007, P. R. China

Mark T. Jarvi

Department of Medical Biophysics, University of Toronto/University Health Network, Toronto, Ontario M5G 1L7, Canada

Received: 28 April 2014

Accepted: 12 August 2014

Published: 29 September 2014

Excited-state singlet oxygen (¹O₂), generated during photodynamic therapy (PDT), is believed to be the primary cytotoxic agent with a number of clinically approved photosensitizers. Its relative concentration in cells or tissues can be measured directly through its near-infrared (NIR) luminescence emission, which has correlated well with in vitro cell and in vivo normal skin treatment responses. Here, its correlation with the response of tumor tissue in vivo is examined, using the photosensitizer meso-tetrahydroxyphenylchlorin (mTHPC) in an animal model comprising luciferase- and green fluorescent protein (GFP)-transduced gliosarcoma grown in a dorsal window chamber. The change in the bioluminescence signal, imaged pretreatment and at 2, 5 and 9 d post treatment, was used as a quantitative measure of the tumor response, which was classified in individual tumors as "non", "moderate" and "strong" in order to reduce the variance in the data. Plotting the bioluminescence-based response vs the ¹O₂ counts demonstrated clear correlation, indicating that ¹O₂ luminescence provides a valid dosimetric technique for PDT in tumor tissue.

Keywords: Photodynamic therapy; singlet oxygen luminescence dosimetry; bioluminescence

Cited by (4):

Defu Chen, Ying Wang, Buhong Li, Huiyun Lin, Xuechun Lin, Ying Gu. (2016) Effects of pulse width and repetition rate of pulsed laser on kinetics and production of singlet oxygen luminescence. Journal of Innovative Optical Health Sciences 09:06. Online publication date: 1-Nov-2016. [Abstract | PDF (336 KB) | PDF Plus (353 KB)]

Buhong Li, Lisheng Lin, Huiyun Lin, Brian C. Wilson. (2016) Photosensitized singlet oxygen generation and detection: Recent advances and future perspectives in cancer photodynamic therapy. Journal of Biophotonics. Online publication date: 1-May-2016. [CrossRef]

Roman Dědic, Adam Stíbal, Vojtěch Vyklický, Miloslav Franěk, Antonín Svoboda, Jan Hála. (2015) Parallel fluorescence and phosphorescence monitoring of singlet oxygen photosensitization in rats. Journal of Innovative Optical Health Sciences 08:06. Online publication date: 1-Nov-2015. [Abstract | PDF (1677 KB) | PDF Plus (568 KB)]

Buhong Li, Brian C. Wilson. (2015) Editorial. Journal of Innovative Optical Health Sciences 08:01. Online publication date: 1-Jan-2015. [Abstract | PDF (59 KB) | PDF Plus (69 KB)]

Read More: http://www.worldscientific.com/doi/abs/10.1142/S1793545815400064