武汉光电论坛第141期

报告题目：功能光学成像，从单分子到人眼

Functional Optical Imaging, From Single Molecules to Human Eyes

时 间：2018年6月8日10:00-12:00

地 点：光电国家研究中心A101

报 告 人：张 浩 教授，美国西北大学

邀 请 人：黄振立 教授

报告人简介：

张浩教授就职于美国西北大学生物医学工程系，他分别于1997年和2000年在上海交通大学获得学士和硕士学位，2006年获得了德州农工大学的博士学位。2006-2007年在华盛顿圣路易斯大学做博士后。他和同事首次实现了光声显微成像技术（(Nature Biotechnology 2006, Nature Protocols 2007, PNAS 2010）和多光谱超分辨成像（Nature Communications 2016），也首次观测到了DNA固有的随机荧光发射的过程（PNAS 2016）。张浩教授先后获得了NSF CAREER award（2010）、NIH Director’s Challenge Award（2010）、NIH IMPACT award（2015）、SPIE Translational Research Award（2016）、US National Academy of Sciences Cozzarelli Prize（2017）。他的研究兴趣包括：OCT、超分辨成像、单分子成像、视觉科学和癌症等。他是Biomedical Optics Express的副主编、NIH 神经科学和眼科成像技术研究部的特许会员。2015年，他联合创办了Opticent Health公司来商业化他们实验室发展的OCT技术。

Biography:

Hao F. Zhang is a Professor of Biomedical Engineering at Northwestern University. He received his Bachelor and Master degrees from Shanghai Jiao Tong University(Shanghai, China) in 1997 and 2000, respectively, and his Ph.D. degree from Texas A&M University (College Station, Texas) in 2006. From 2006 to 2007, he was a postdoctoral fellow at Washington University in St. Louis. He and colleagues reported the first demonstration of photoacoustic microscopy (Nature Biotechnology 2006, Nature Protocols 2007, PNAS 2010), the first spectroscopic super-resolution imaging (Nature Communications 2016), and the first observation of intrinsic stochastic fluorescence emission from DNA (PNAS 2016). He received the NSF CAREER award and NIH Director’s Challenge Award in 2010, the NIH IMPACT award in 2015, the SPIE Translational Research Award in 2016, and the US National Academy of Sciences Cozzarelli Prize in 2017. His research interests include optical coherence tomography, super-resolution imaging, single molecule imaging, vision science, and cancers. He is an associate editor of Biomedical Optics Express and is a chartered member of the NIH Neuroscience and Ophthalmic Imaging Technologies (NOIT) study section. In 2015, he co-founded Opticent Health to commercialize optical coherence tomography technologies developed in his lab.

报告摘要：

我的实验室主要研究两种光学成像技术：OCT和光子定位显微成像，以填补临床诊断和基础生物医学研究的空白。我们发展了可见光OCT（vis-OCT）技术，用于提取比高质量解剖成像更多的病理和生理学信息。vis-OCT工作在可见光谱范围内，在超高分辨率成像、血管造影、氧代谢成像和超微结构病理传感等方面表现出了巨大的潜力。目前，我们将vis-OCT技术应用于几种致盲性疾病（糖尿病视网膜病变、视网膜静脉阻塞、黄斑变性和青光眼）和脑部疾病（缺血性中风和脑瘤）的研究。

在超分辨成像研究中，我们发展了多光谱光子定位成像技术（SPLM）。传统的光子定位成像通过分析单个分子随机发射的光子的空间分布特征，来重建超分辨光学图像。SPLM还捕获了这些光子固有的光谱特征。通过分子识别和复原，在不显著增加图像帧数的前提下，SPLM可以达到10nm甚至更高的分辨率。利用SPLM，我们可以进行多分子超分辨成像，可以使用发射光谱只有细微差别的荧光标记物。我们还探究了无标记核苷酸固有的随机荧光发射，用于免标记的超分辨成像。

Abstract:

My lab focuses on two optical imaging technologies, optical coherence tomography (OCT) and photon localization microscopy, to fill the gaps in both clinical diagnoses and fundamental biomedical investigations. To enable OCT to extract physiological and pathological information beyond high-quality anatomical imaging, we developed visible-light OCT or vis-OCT. Operating within the visible-light spectral range, vis-OCT has demonstrated great potential in ultra-high resolution imaging, angiogram, oxygen metabolic imaging, and ultrastructural pathological sensing. We are applying vis-OCT to investigate several blinding diseases (diabetic retinopathy, retinal vein occultation, macular degeneration, and glaucoma) and brain disorders (ischemic strokes, brain tumors).

In our super-resolution imaging work, we developed spectroscopic photon localization microscopy(SPLM). Traditional photon localization microscopy analyzes the spatial distributions of photons emitted stochastically by individual molecules to reconstruct super-resolution optical images. SPLM further captures the inherent spectroscopic signatures of these photons. Through molecular discrimination and regression, SPLM can reach the spatial resolution of 10 nm or greater without significantly increasing the total number of image frames. Using SPLM, we demonstrated simultaneous multi-molecular super-resolution imaging, where the number of fluorescence labels can have largely overlapping emission spectra with only minute differences. We further investigated intrinsic stochastic fluorescence emission from unstained nucleotides, seeking label-free super-resolution imaging.

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